8th Cuban Congress on Microbiology and Parasitology, 5th National Congress on Tropical Medicine and 5th International Symposium on HIV/aids infection in Cuba

Title

INTRODUCTION OF A TECHNIQUE FOR Salmonella spp. PRESUMPTIVE IDENTUFICATION IN WATER AND FOOD SAMPLES

Authors

Hermes Fundora Hernández , Giselle Pereda Lamela , Virginia Leyva Castillo , María Isabel González González

Abstract


Introduction: The Salmonella gender is one of those more widely studied among the patogens that can be isolated in foods and water. There are situations in which it becomes necessary to carry out a presumptive diagnosis in few hours for the decisions taking in public health. Objectives: To validate under our work conditions a commercial method (RIDASCREEN Salmonella) by ELISA technique and to propose an analytic new procedure for Salmonella spp. Identification in water and foods. Materials and Methods: RIDASCREEN Salmonella, r-biopharm, Darmstadt, Germany commercial package was used. We were determined the precision, the possible existence of crossed reaction with a microorganism that presents antigenic likeness with Salmonella spp. gender, the sensibility and specificity, as well as the positive and negative predictive values. Results: The agreement was of 100% in the three series of dilutions used to evaluate the precision of the method. We didn't find the existence of crossed reaction with none of the dilutions used of Citrobacter freundii. For water samples it was obtained a sensibility of 100%, specificity of 87,5%; positive predictive value of 0,96 and predictive negative value of 1,0. For the food samples it was obtained a sensibility of 77,7%; specificity of 70,4%, predictive positive value of 0,30 and predictive negative value of 0,95. We intended a procedure that constitutes a modification of the traditional one used for determination of Salmonella spp. Presence water samples. Conclusions: It was validated under our work conditions an ELISA technique for Salmonella spp. identification in water and food samples. The obtained results allow to affirm that it is about a diagnostic useful package and cash for the initial presumptive diagnosis of samples coming from sprouts and conflicts in the case of the waters and not useful in the case of foods. An analytic new procedure was elaborated for Salmonella spp. Identification in water samples.

References


1.- Ryan KJ, Ray CG. Enterobacteriaceae. In: Ryan KJ; Ray CG, editors. Sherris Medical Microbiology. New York: McGraw Hill; 2004. p. 324-49.

 

2.- Dapena MV. Enterobacterias. En: Llops A, Valdés – Dapena M, Zuaso, editores. Microbiología y Parasitología Médicas. La Habana: Ecimed; 2001 .p. 251-280.

3.- Su LH, Chiu CH. Salmonella: clinical importance and evolution of nomenclature. Chang Gung Med J 2007; 30(3):210-9.

4.- Poppof Y, Bockemuhl J, Brenner FW, Gheesling LL. Supplement 2000 (no 44) to the Kauffmann-White scheme. Resa Microbiol 2001; 152 (10): 907- 9.

 

5.- Gatto AJ, Peters TM, Green J, Fisher IS, Gill ON, O'brien SJ, et al. Distribution of molecular subtypes within Salmonella enterica serotype Enteritidis phage type 4 and S. Typhimurium definitive phage type 104 in nine European countries, 2000-2004: results of an international multi-centre study. Epidemiol Infect 2006; 134(4):729-36.

6.- Deb M, Kapoor L. Salmonella nomenclature seen in the literature. Indian J Med Microbiol 2005; 23(3):204-5.

7.- Heyndrickx M, Pasmans F, Ducatelle R, Decostere A, Haesebrouck F. Recent changes in Salmonella nomenclature: the need for clarification. Vet J 2005; 170(3):275-7.

8.- Tindall BJ, Grimont PA, Garrity GM, Euzéby JP. Nomenclature and taxonomy of the genus Salmonella. Int J Syst Evol Microbiol 2005; 55(1):521-4.

9.- Autoridad Europea de Seguridad Alimentaria. Informe de la EFSA-ECDC sobre intoxicaciones alimentarias en la UE. EFSA Journal 2011; 9 (3): 2090-8.

10.- Puig Peña Y, Espino Hernández M, Leyva Castillo V, Aportela López N, Machín Díaz M. Serovariedades y patrones de susceptibilidad a los antimicrobianos de cepas de Salmonella aisladas de alimentos en Cuba. Rev Panam Salud Pública 2011; 30(6):561–5.

11.- Dunn G, Bakker K, Harris L. Drinking Water Quality Guidelines across Canadian Provinces and Territories: Jurisdictional Variation in the Context of Decentralized Water Governance. Int J Environ Res Public Health 2014;11(5):4634-51.

12.- Ivanova LV, Artemova TZ, Gipp EK, Zagaĭnova AV, Maksimkina TN, Krasniak AV, et al. Harmonization of microbiological and parasitological indices of epidemic safety of drinking water with the international requirements. Gig Sanit 2013;(5):45-8.

13.- Fundora Hernández H, Puig Peña Y, Chiroles Rubalcaba S, Rodríguez Bertheau AM, Gallardo Díaz J, Milián Samper Y. Métodos inmunológicos para la identificación rápida de bacterias y protozoos en aguas. Revista Cubana de Higiene y Epidemiología 2013; 51(1): 0-0.